Subtracting infinity from infinity lacks mathematical meaning unless contextualized within limits or orders of magnitude. In cardinal numbers, operations like addition can be defined, such as card(R) + card(N) = card(R) and card(N) + card(N) = card(N). The concept of infinity varies, with different interpretations in the extended real line and hyperreal numbers, where subtraction may be defined under specific conditions. However, in standard mathematics, infinity is not treated as a real number, making operations like subtraction undefined. The discussion emphasizes the need for clarity regarding the type of infinity and the mathematical framework being used.