A Question On Electron Microscopy (TEM)

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SUMMARY

The discussion focuses on calculating the number of fields required to cover a specific area using Transmission Electron Microscopy (TEM) at a magnification of 100,000. The user seeks to determine how many fields are necessary to cover 0.1 mm² and what percentage of the original 1 cm³ biological sample this represents. Key factors include the resolution of the TEM, which is influenced by the wavelength and energy of the electron beam. The user also inquires about the definition of a "field" in this context.

PREREQUISITES
  • Understanding of Transmission Electron Microscopy (TEM) principles
  • Knowledge of magnification calculations
  • Familiarity with biological sample preparation techniques
  • Basic mathematical skills for area and percentage calculations
NEXT STEPS
  • Research the calculation of fields of view in TEM at various magnifications
  • Learn about the relationship between resolution and electron beam energy in TEM
  • Explore methods for calculating the volume percentage of sampled areas
  • Investigate the significance of field size in microscopy applications
USEFUL FOR

Students and researchers in microscopy, particularly those studying biological samples, as well as professionals involved in electron microscopy techniques and calculations.

kranav
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Homework Statement



Hello!
The question is as follows:

1. You are provided with a 1 cm3 of biological sample from which 0.1 mm thin sections are cut. A search for virus cells at a magnification of 100,000 has been proposed. The fluorescent screen on which the image is to be projected has a diameter of 15 cm.

(a) How many fields need to be searched to cover an area of 0.1 mm2?

(b) What percentage of the original sample does the searched volume represent?

(c) Comment on the significance of the calculated values.


Homework Equations





The Attempt at a Solution



I don't know of any formulas for this question.
The magnification might be a key here.
Can I find the resolution of the TEM using this?
or maybe the max distance squared I get at 100000 mags (one field) and then use that to calculate the total number of fields to cover 0.1 mm2 ?

In the second part I can do the same (find the area at 100000 mags) and get the percentage.

thanks.
 
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What is meant by field ?
Resolution depends on the wavelength/energy of electron beam used in TEM.
 

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