If you want a proof from basic definitions, that's a long proof!
One way to define the "natural numbers" is to use the Peano axioms:
The natural numbers consist of a set N, together with a function, s (called the "successor function") from N to N such that
1) There exist a unique member of N, called "1" such that s is a one-to-one function from N to N-{1}. (In other words, every natural number has a successor, every natural number except 1 is the successor of some natural number.)
2) If X is a set of natural numbers such that 1 is in X and, whenever n is in X so is s(n), then X is the set of all natural numbers. (This is the "induction axiom.)
We can define ">" by "m> n if and only if there exist a natural number x such that x+ n= m.
From there we can define "m+ n", for m and n natural numbers, by
1) 1+ n= s(n).
2) if m\ne 1, then there exist x such that m= s(x) and we define
m+ n= s(x+ n).
One needs to show that is "well defined". That is, that given any m,n in N, there exist a unique member of N equal to m+n.
Once we have that, we can define n- m as the unique number, x, such that m+ x= n.
Now, if n> 1, by definition of ">", there exist k such that k+1= n and therefore, n- k= 1.
(micromass got in ahead of me again!)