Buffer solution working with DNA -- I have to dissolve dried oligos in PBS

AI Thread Summary
To dissolve dried oligonucleotides in PBS (10mM phosphate buffer, 100mM NaCl, pH 7.4), it is essential to prepare the buffer correctly. The process involves mixing specific amounts of sodium phosphate dibasic and sodium phosphate monobasic to achieve the desired pH, along with adding sodium chloride for ionic strength. Detailed instructions for preparing PBS at pH 7.4 can be found in the linked article, which provides a comprehensive guide on the necessary components and their concentrations. Proper preparation of PBS is crucial for effective oligo dissolution and subsequent experiments.
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This is the first time I am working with DNA and I have to dissolve the dried oligos in PBS( 10mM phosphate buffer, 100mM NaCl, ph=7.4) buffer. However I don't understand how to do that. I will really appreciate if somebody can please explain me. Thank you in advance.
 
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