Buffer solution working with DNA -- I have to dissolve dried oligos in PBS

Click For Summary
To dissolve dried oligonucleotides in PBS (10mM phosphate buffer, 100mM NaCl, pH 7.4), it is essential to prepare the buffer correctly. The process involves mixing specific amounts of sodium phosphate dibasic and sodium phosphate monobasic to achieve the desired pH, along with adding sodium chloride for ionic strength. Detailed instructions for preparing PBS at pH 7.4 can be found in the linked article, which provides a comprehensive guide on the necessary components and their concentrations. Proper preparation of PBS is crucial for effective oligo dissolution and subsequent experiments.
chmenaive
Messages
1
Reaction score
0
This is the first time I am working with DNA and I have to dissolve the dried oligos in PBS( 10mM phosphate buffer, 100mM NaCl, ph=7.4) buffer. However I don't understand how to do that. I will really appreciate if somebody can please explain me. Thank you in advance.
 
Chemistry news on Phys.org

Thread 'Flash Joule Heating (FJH) Method for treating process waste streams'

I caught the tail end of a video about a new application for treating chemical or process waste, which is applied to 'red' mud or contaminated bauxite residue, but the person of interest mention recovering critical minerals from consumer electronics, as well as treating mine tailings and processing ores of rare earth elements. What I found so far is the following: New electrical flash method rapidly purifies red mud into strong ceramics, aluminum feedstock...
View full post »

Similar threads

How can I prepare a 0.1M NaOAc buffer at pH 5 using titration?
  • · Replies 4 ·
Replies
4
Views
5K
Perfect Espresso Water Chemistry
  • · Replies 7 ·
Replies
7
Views
4K
How to create a buffered pH gradient?
  • · Replies 9 ·
Replies
9
Views
9K
CO2 bubbling in phosphate buffered saline (pH 7.4)
  • · Replies 1 ·
Replies
1
Views
6K
How Does Temperature Affect pKa Values in Buffer Preparations?
  • · Replies 3 ·
Replies
3
Views
4K
Interpreting buffer solution reaction
  • · Replies 2 ·
Replies
2
Views
3K
Buffer solutions and pKa confusion
  • · Replies 3 ·
Replies
3
Views
2K
Buffer Solutions: Weak Acid & Conjugate Base or Vice Versa?
  • · Replies 7 ·
Replies
7
Views
6K
How Do I Create a Sodium Acetate Buffer Solution with a Specific pH?
  • · Replies 3 ·
Replies
3
Views
26K
Solution Potential Witout Electrodes (Pourbaix diagram help)
  • · Replies 1 ·
Replies
1
Views
3K