Calibration curve and concentration?

Click For Summary

Discussion Overview

The discussion revolves around creating calibration curves for calcium and sodium based on absorbance data obtained from AAS/FES water analysis. Participants seek clarification on how to determine concentration from the provided data and the relationship between absorbance and concentration.

Discussion Character

  • Homework-related
  • Technical explanation

Main Points Raised

  • One participant asks for help in creating calibration curves and determining concentration from absorbance values.
  • Another participant suggests that the x-axis should represent concentration and the y-axis should represent absorbance.
  • A participant questions how to find the concentration, indicating potential confusion about the data provided.
  • Another participant points out that the concentration values are already given for the standards, implying that the calibration curves should be based solely on these standards.
  • A later reply confirms that the calibration curves will only include the standard data provided.

Areas of Agreement / Disagreement

Participants generally agree that the calibration curves will be based on the standard data, but there is some confusion regarding the process of determining concentration from the absorbance values of the samples.

Contextual Notes

The discussion does not clarify the specific method for deriving concentration from the calibration curves once they are established, leaving some steps unresolved.

mimibajiduh
Messages
15
Reaction score
0
calibration curve and concentration??

The following is all of the data I have from a AAS/FES for water analysis. I need to make the calibration curves for calcium and sodium. and then I have to find the concentration. can someone please help me with this? i know that the Abs values will be one of my axes but I am confused on what my other axes would be. any help will be appreciated!

Calcium (422 nm/2)

Standards: Abs:
10ppm 0.06
25ppm 0.132
50ppm 0.245
75ppm 0.375
100ppm 0.44
Sample: Abs:
Outside Tap 0.295
Kitchen Tap 0.31
Hose Tap 0.23
Drinking Tap 0.23
Kress 0.061
Oaks Kitchen 0.145
Barkley Fountain 0.15
La Fetra Fountain 0.165

Sodium (589 nm/2)

Standards: Abs:
1ppm 0.09
5ppm 0.345
10ppm 0.525
25ppm 0.848
50ppm 1.275
75ppm 1.52
Sample: Abs:
Outside Tap 0.841
Kitchen Tap 0.762
Hose Tap 0.944
Drinking Tap 0.944
Kress 0.034
Oaks Kitchen 1.248
Barkley Fountain 1.208
La Fetra Fountain 1.208
 
Physics news on Phys.org


x - concentration, y - absorbance.

--
methods
 


how do I find the concentration?
 


It is given for your standards, isn't it? Or are you looking at something different from what you have posted?

--
 


ohhhhh! so my calibration curves are only going to be of the standards, right? because everything that I posted is all the data I have.