Work and potential energy are interconnected through the Work-Energy Theorem, which states that work done on a system results in a change in kinetic energy (W = ΔKE). Negative work occurs when a system loses energy, such as when a force opposes motion, leading to a decrease in kinetic energy. This relationship is expressed in the conservation of energy equation, ΔKE + ΔPE = 0, indicating that a decrease in kinetic energy corresponds to an increase in potential energy. The negative sign in the integral of electric field relates to the convention that energy lost by the system results in negative work. Understanding these concepts clarifies the relationship between work, kinetic energy, and potential energy in physics.