Cell cryoperservation with DMSO

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DMSO does not replace water in cells, contrary to some claims. Research indicates that DMSO can affect cell membranes by thinning the lipid bilayer and potentially creating transient holes at certain concentrations. High concentrations of DMSO can even damage the membrane. Its primary function appears to be preventing cell damage by alleviating osmotic stress and inhibiting the formation of ice crystals, rather than substituting for water. The consensus in scientific literature supports the view that DMSO aids in preserving cell integrity during freezing processes without replacing water.
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So my question is this does DMSO as someone in the lab I am working at says "it replaces the water in the cell" is that true??

From what I have read in journals a couple of journal articles they never said anything about it replacing the water. It did talk about thinning of the bilayer and at certain concentrations starting to form transient holes in the bi layer and even destroying the membrane at high enough concentrations. I was under the assumption that the DMSO helps prevent cell damage by helping relive osmotic stress and helping the water not to form crystals that damage the membrane. I'm guessing there is more to it than that but the idea that it totally replaces the water I wasn't to sure about and have yet to find a definitive answer.
 
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My understanding is that DMSO prevents the nucleation of ice crystals. It does not "replace" water.
 

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