Concentration from density and purity

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Discussion Overview

The discussion revolves around the calculation of molarity and dilution for a solution of 1-thioglycerol, focusing on the relationship between density, purity, and the desired final concentration. Participants explore the implications of their calculations and the accuracy of the instructions provided.

Discussion Character

  • Homework-related
  • Mathematical reasoning
  • Debate/contested

Main Points Raised

  • One participant calculates the molarity of the stock solution to be 11.5 M based on the density and molar mass, but questions the validity of the dilution instructions provided.
  • Another participant seeks clarification on whether the desired concentration refers to hydroxyl groups, suggesting a potential misunderstanding of the target concentration.
  • A participant confirms that they are aiming for a concentration of 1.5 x 10^-4 M of monothioglycerol, not hydroxyl groups.
  • One participant re-evaluates the calculations for the final concentration, detailing the steps to determine the grams needed and the volume required from the stock solution, while expressing uncertainty about the use of density in their calculations.

Areas of Agreement / Disagreement

Participants generally agree on the calculations presented, but there is uncertainty regarding the interpretation of the desired concentration and the accuracy of the dilution instructions. The discussion remains unresolved regarding the correct approach to achieve the target concentration.

Contextual Notes

Participants express uncertainty about the correct application of density in their calculations and the implications of the purity of the stock solution on the final concentration.

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Homework Statement


This isn't homework, rather it's regular work. I have a >97% solution of 1-thioglycerol, the density of which is 1.25 g/mL. I am trying to get a 1.5 x 10-4 M solution at the end, and I suspect the instructions I have been given are incorrect.
Molar mass = 108.16

Homework Equations


Concentration = Density / molar mass.

The Attempt at a Solution


The molarity of my stock solution is (1.25 * 1000) = 1250 g/L. 1250 / 108.16 = 11.5 M.
I am instructed to make a 10-fold dilution, giving me a molarity of 1.15 M.
I should then take 6.5 microlitres and add this to a final volume of 100 millilitres.
But 1.15 x (6.5 x 10^-6) = 7.5 x 10^-6. Divided by (100 x 10^-3) = 7.5 x 10^-5 M, which is half the concentration I need?

Thanks, Adam.
 
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Are you looking for a solution that is 1.5 x 10-4 M in hydroxyl groups (2 per molecule)?
Otherwise I agree with your calculation.
 
No, I'm looking for 1.5 x 10-4 M monothioglycerol.
 
I calculated this again from the end concentration, and would just like anyone to proof-check it.
If we start with the concentration I want at the end (1.5 x 10^-4 moles per litre), and if I want this in a volume of 100 millitlitres:
(1.5 x 10^-4) x (0.1) = 1.5 x 10^-5 moles.
Multiply the moles needed by the molecular mass to obtain the grams needed: (1.5 x 10^-5) x 108.16 = 0.00162 grams.
The density is 1.25 grams per millilitre, but we need the grams per litre amount, so 1.25 x 1000 = 1250 grams per litre.
Divide the grams by the density in grams per litre to obtain the volume needed: 0.00162 / 1250 = 1.3 x 10^-6 L.
This is 1.3 microlitres. Therefore, if I makea 10-fold dilution of my stock solution, I should need 13 microlitres per 100 millilitres of media to make.
This is the same answer as I got in my original post, but I'm uncertain if I've used the density correctly...
 

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