Concentration from density and purity

AI Thread Summary
The discussion revolves around preparing a 1.5 x 10^-4 M solution of 1-thioglycerol from a >97% stock solution with a density of 1.25 g/mL. The calculated molarity of the stock solution is 11.5 M, leading to a 10-fold dilution resulting in 1.15 M. The participant confirms that to achieve the desired final concentration in 100 mL, 1.3 microlitres of the diluted solution is needed, which equates to 13 microlitres of the stock solution after dilution. There is uncertainty regarding the correct application of density in the calculations, but the participant's results remain consistent throughout. The focus is on ensuring the accuracy of the dilution process to achieve the target concentration.
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Homework Statement


This isn't homework, rather it's regular work. I have a >97% solution of 1-thioglycerol, the density of which is 1.25 g/mL. I am trying to get a 1.5 x 10-4 M solution at the end, and I suspect the instructions I have been given are incorrect.
Molar mass = 108.16

Homework Equations


Concentration = Density / molar mass.

The Attempt at a Solution


The molarity of my stock solution is (1.25 * 1000) = 1250 g/L. 1250 / 108.16 = 11.5 M.
I am instructed to make a 10-fold dilution, giving me a molarity of 1.15 M.
I should then take 6.5 microlitres and add this to a final volume of 100 millilitres.
But 1.15 x (6.5 x 10^-6) = 7.5 x 10^-6. Divided by (100 x 10^-3) = 7.5 x 10^-5 M, which is half the concentration I need?

Thanks, Adam.
 
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Are you looking for a solution that is 1.5 x 10-4 M in hydroxyl groups (2 per molecule)?
Otherwise I agree with your calculation.
 
No, I'm looking for 1.5 x 10-4 M monothioglycerol.
 
I calculated this again from the end concentration, and would just like anyone to proof-check it.
If we start with the concentration I want at the end (1.5 x 10^-4 moles per litre), and if I want this in a volume of 100 millitlitres:
(1.5 x 10^-4) x (0.1) = 1.5 x 10^-5 moles.
Multiply the moles needed by the molecular mass to obtain the grams needed: (1.5 x 10^-5) x 108.16 = 0.00162 grams.
The density is 1.25 grams per millilitre, but we need the grams per litre amount, so 1.25 x 1000 = 1250 grams per litre.
Divide the grams by the density in grams per litre to obtain the volume needed: 0.00162 / 1250 = 1.3 x 10^-6 L.
This is 1.3 microlitres. Therefore, if I makea 10-fold dilution of my stock solution, I should need 13 microlitres per 100 millilitres of media to make.
This is the same answer as I got in my original post, but I'm uncertain if I've used the density correctly...
 
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