To develop a test for detecting prions in human cells without prior knowledge of their structure or function, initial steps involve researching existing detection methods. Key approaches include traditional histological techniques, genetic assays for hereditary prion diseases like Creutzfeldt-Jakob disease (CJD), and newer detection methods highlighted in recent literature. Histological methods typically involve staining techniques to visualize prion aggregates in tissue samples. Genetic assays focus on identifying mutations associated with hereditary prion diseases through DNA analysis. Additionally, emerging methods, such as urine-based tests, are being explored for their potential to detect prions non-invasively. These resources provide a foundational understanding for further exploration into prion detection.
