Factors affecting beet root cell membrane

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spikeyshot
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Hi. I am currently doing an experiment regarding to the factors affecting beet root cell membrane. Some of them are pH (2,4,6,8,10) , Ethanol (1%, 25%, 50%) and Detergent(1%, 5%). I've seen that different pH, different concentration in ethanol and detergent each has different results in both terms of color and distance. I am wondering how this could happen. And it seems like the more the pH "get closer" to pH 7, the shorter the distance and the less concentrated the color. Can anybody help me with these?
 
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