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Warpspeed13
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Would the methods used to create glow in the dark e.coli readily work on other bacteria?
Here's the procedure http://www.apsnet.org/edcenter/K-12/TeachersGuide/PlantBiotechnology/Documents/TransformationEcoliBacterium.pdfSimon Bridge said:What no plasmid? - do you have a reference?
All the ones I've seen are like this:
http://grad.bio.uci.edu/ecoevo/ahebling/Research/GK-12_files/GlowingBacteria.pdf
... and that is pretty much what the New Scientist article is talking about.
Warpspeed13 said:Do you know where the derivative Dolores can be purchased? I want to take pond scum from a local lake and make a glowing variety for a freshwater biome
Cool thanks, are you by any chance on the bungie forums?Ygggdrasil said:Note that most of the organisms commonly referred to as pond scum are not bacteria, but algae, a diverse group of unicellular eukaryotic organisms. While it is certainly possible to introduce fluorescent transgenes into these organisms, the procedures may be different from those used to modify bacteria. However, because there has been considerable research into modifying algae for biofuel applications, there may be some published, standard procedures for introducing transgenes into algae.
From your reference:Here's the procedure http://www.apsnet.org/edcenter/K-12/...iBacterium.pdf
Warpspeed13 said:Cool thanks, are you by any chance on the bungie forums?
Glow in the dark E.Coli is a type of bacteria that has been genetically modified to produce a fluorescent protein, causing it to emit a greenish glow under certain conditions.
Glow in the dark E.Coli is created through genetic engineering techniques, where scientists insert DNA from bioluminescent organisms into the bacteria's genome. This allows the bacteria to produce the necessary proteins for glowing.
Glow in the dark E.Coli is generally considered safe, as it is a non-pathogenic strain of the bacteria. However, precautions should still be taken when handling it in a laboratory setting.
Glow in the dark E.Coli has potential applications in various fields, including biotechnology, environmental monitoring, and medical research. It can also be used as a tool for visualizing and studying bacterial growth and behavior.
The duration of the glow in glow in the dark E.Coli depends on factors such as the strength of the light source used to activate it and the concentration of the fluorescent protein produced. In general, the glow can last for several hours to a few days.