How do you calculate the concentration of nucleotides from DNA?

  • Thread starter Thread starter nancy189
  • Start date Start date
  • Tags Tags
    Concentration
AI Thread Summary
To calculate the concentration of nucleotides from the concentration of DNA, one must multiply the DNA concentration by the number of nucleotides per DNA molecule. For instance, a 1nM solution of a 100 base pair double-stranded DNA would yield a nucleotide concentration of 200nM, as each base pair consists of two nucleotides. This indicates that DNA and nucleotide concentrations are not the same, and the conversion depends on the length of the DNA strand.
nancy189
Messages
8
Reaction score
0
Hi,

I was wondering if given the concentration of DNA, how does one calculate the concentration of nucleotides? Are they the same thing?

Thanks.
Nancy
 
Biology news on Phys.org
The concentration of nucleotides would just be the concentration of DNA multiplied by the number of nucleotides per molecule of DNA. For example a 1nM solution of a 100bp piece of double-stranded DNA would have a nucleotide concentration of 200nM.
 

Thread 'Chagas disease now established in US'

Chagas disease, long considered only a threat abroad, is established in California and the Southern U.S. According to articles in the Los Angeles Times, "Chagas disease, long considered only a threat abroad, is established in California and the Southern U.S.", and "Kissing bugs bring deadly disease to California". LA Times requires a subscription. Related article -...
View full post »

Thread 'I need help understanding this quote about recent Natural Selection'

I am reading Nicholas Wade's book A Troublesome Inheritance. Please let's not make this thread a critique about the merits or demerits of the book. This thread is my attempt to understanding the evidence that Natural Selection in the human genome was recent and regional. On Page 103 of A Troublesome Inheritance, Wade writes the following: "The regional nature of selection was first made evident in a genomewide scan undertaken by Jonathan Pritchard, a population geneticist at the...
View full post »

Thread 'Fermentation using mixed legume and grain feedstock'

I use ethanol for cleaning glassware and resin 3D prints. The glassware is sometimes used for food. If possible, I'd prefer to only keep one grade of ethanol on hand. I've made sugar mash, but that is hardly the least expensive feedstock for ethanol. I had given some thought to using wheat flour, and for this I would need a source for amylase enzyme (relevant data, but not the core question). I am now considering animal feed that I have access to for 20 cents per pound. This is a...
View full post »

Similar threads

Does DNA get affected by the concentration of the liquids around it?
Replies
5
Views
1K
Medical Biochemistry (Nucleotide Polymerization)
Replies
5
Views
2K
Can a Single Nucleotide Mutation Significantly Change a Protein's Function?
Replies
4
Views
2K
What is the role of hydroxylapatite in the formose reaction and abiogenesis?
Replies
13
Views
2K
Semi-synthetic organism with an expanded genetic code
Replies
8
Views
5K
COVID Plasmid DNA from Pfizer COVID Vaccine Manufacturing
Replies
3
Views
2K
Biological Info: Understanding & Analyzing
Replies
15
Views
3K
Expert Answer: The Importance of Research and Self-Learning in Asking Questions
Replies
4
Views
3K
Fossil origin based on DNA testing
Replies
15
Views
3K
[Physiology] Intuition behind the Nerst equation
Replies
6
Views
2K

Hot Threads

Recent Insights

Back
Top