How to Calculate Transformation Efficiency?

AI Thread Summary
Transformation efficiency can be calculated using the formula: colonies on the plate divided by the amount of DNA plated in micrograms. There is uncertainty about how to accurately measure the amount of DNA plated, which is crucial for the calculation. A reference website suggests that transformation efficiency should yield a high value, around 10^9, but the user is unsure if their results align with this expectation. The user expresses concern that their experimental outcomes may not have been successful, potentially affecting their efficiency calculations. Accurate measurement and successful experimental conditions are essential for determining transformation efficiency correctly.
higherme
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does anyone know how to calculate the Transformation efficiency?
 
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actually I kind of have the formula of the transformation efficiency

colonies on plate/ amount of DNA plated in (ug)

I just don't know how to find the amounf of DNA plated... well i kind of have an answer
but it's really small... but this website saids that the the transformation efficiency should be a big number... something like 10^9 that they got from their example...

so, i don't know if i am doing it right...
 
or maybe it's because my experient didn't work out that great
 

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