MATLAB imports audio files with mono sound as an m x 1 array and stereo sound as an m x 2 array, where m represents the total number of samples. Users can segment these audio files into shorter clips by manipulating the array dimensions. After segmenting, the shorter audio clips can be saved using the wavwrite function, which allows for easy re-exporting of the modified audio data. For detailed guidance, refer to the MATLAB documentation on importing and exporting audio files.