How would i go about breaking an isopeptide bond?

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The discussion centers on the formation of peptide bonds between the carboxyl group and the amino acid lysine. An enzyme is needed for this process, and while proteases are suggested as suitable enzymes, it is noted that hydrochloric acid and heat could also facilitate the reaction. For a lysine-specific protease, a link to a specific product is provided. Ultimately, the original poster decides to use trypsin, which is readily available at a local health store, to achieve their goal.
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Hi. i don't know a lot about chemistry so bear with me.
the bond is between a member of the carboxyl group and the amino acid lysine.
as i understand i need an enzyme. what readily available enzyme would suffice?
would protease work?
 
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Your question doesn't make sense as presented. The peptide bond is between the amino functionality of an amino acid (lysine?) and the carboxy functionality of a different amino acid (also lysine?). The proteases as a class would be the enzyme you are looking for but HCl/heat would work as well. If you are looking for a lysine (lys) specific protease at the end of a peptide, http://www.roche-applied-science.com/pack-insert/1420429a.pdf" would work.
 
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nevermind. i got what i want. trypsin from the local health mart.
 

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