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Ideas, please

  1. Mar 22, 2005 #1
    • Why should some Poly-A sequences lay upstream of a reporter gene?
    • Isogenic cell lines?
    • Null cell lines?

    Any ideas are greatly appreciated.
     
  2. jcsd
  3. Mar 22, 2005 #2

    iansmith

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    You should find the answere if ask yourself: What is the role of the polyA sequence/tail during eukaryote DNA transcription?


    Isogenic means similar genome/genotype.

    A null mutant usually lack a specific trait.
     
  4. Mar 23, 2005 #3
    Usually the poly A sequence lays downstream of a gene to give it good RNA termination and translation. BUt when it lies upstream is something new :bugeye: . Upstream means before the promoter. How come?
     
  5. Mar 23, 2005 #4

    iansmith

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    Is the polyA upstream of the promoter for the reporter gene (lacZ) or upstream of selection marker (noemycin resistance) promoter.

    The reporter gene will be inserted in the gemone and will cut the gene X and the normal polyA will not protetect the fused protein. The poly-A upstream of the selection marker promoter is there to protect the fused protein (geneX-LacZ). The neomycin resistant marker will fused with the rest of the protein and the mRNA will be protect by the geneX polyA tail.
     
  6. Mar 23, 2005 #5
    The polyA is upstream of the promoter for the reporter gene

    Can you explain more of this?

    Where did you get this?

    Thanks.
     
  7. Mar 23, 2005 #6

    iansmith

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