To create a mobile ion cloud in a 5mm square plastic tube over a distance of 10mm in air, one must consider the ionization energy of nitrogen and other relevant molecules. The ions, once ionized, can be moved using an electric field generated by a parallel plate capacitor. Gauss's law can be applied to calculate the electric field between the plates, which can then be used to determine the necessary voltage. Alternatively, experimental methods could also be employed to ascertain the required voltage for ionization. Understanding these principles is crucial for effectively generating and manipulating ion clouds in a controlled environment.