Hi all, I have just done a liquid seperation of B-carotene and Chlorophyll-a and put the 2 samples through a UV spectrometer. The result that I get from the meter for B-carotene is 448nm for 1st peak and 475.1nm for 2nd peak. For Chlorophyll-a my peaks are at 417.9nm and 666.1nm. But I have a few questions here. Why are there 2 peaks for each of them? Is it normal? Is it due to the presence of impurities? Is it due to me not choosing the appropriate blank for the UV spectrometery test? For the case of B-carotene, the 2 peaks are so close together which means they have really poor resolution. Is it normal? Thanks in advance for anyone who can offer me a little guidance. Regards, dibilo.