Multimers :(( I have been trying this thing for past 2-3 weeks with no luck :(( I am trying to make a multimer from an oligo. This is how i did: - Annealed the oligos - Phosphorylated the sample (5' ends) - ligated o/n when i ran it on agarose it shows only a dimer. what can be the reason ? any ideas ?????? Should i use some different protocol ??