What Does a Negative Absorbance Reading Indicate?

AI Thread Summary
A negative absorbance reading typically indicates that the spectrometer is not properly calibrated, suggesting that the measurement may not be accurate. This could also imply the presence of stimulated emission from the sample being analyzed. Users express confusion over why the spectrometer does not simply indicate that the reading is out of range. Proper calibration is essential, similar to setting a zero point on a balance scale. Understanding these readings requires consulting the spectrometer's manual for clarification on measurement protocols.
rick112
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hi

i know this is kind of a basic question..but what in reality does a negative absorbance reading means?? why can't the spectrometer just say its out the reading range or something??
does a negative reading really say something can we look at it and detect something...like if the negative reading is x that means it has so much of some component...
 
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This sounds like a question for the spectrometer's manual perhaps...?
 
that means your measurement is not well calibrated. a negative absorption from an excited sample would indicate stimulated emission
 
It seems like the spectrometer needs to be calibrated. It's like pushing the zero or tare on a balance. You manually set which point is called zero and the device reads other values with respect to that.
 
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