You can calculate the cutoff frequency directly (assuming diffraction-limited performance, etc.) with the information you already have- the entrance pupil diameter 'd' is given by the focal length and f/#, and the cutoff frequency for coherent imaging is f_c = d/(2*λ*f), where λ is the wavelength and f the focal length of the lens. For incoherent imaging, the cutoff frequency is twice that of coherent imaging.
Measuring the OTF directly from an image requires some care, but is straightforward. The procedure I use is to image a sharp edge (a sharp black-to-white transition) and differentiate the image to obtain the line spread function. The OTF (in one direction) is the Fourier transform of the LSF, but the specific frequency values (in 1/pixels) will scale with how many image pixels you used. Correct for that, and then knowing the pixel size, you can obtain the OTF in image space (in, for example, cycles/mm). If you then want the OTF in object space ('resolution limits'), you need to know the magnification of the lens.
Does this help?