Using Biuret Solution to Quantitatively Measure Proteins

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SUMMARY

The discussion centers on the use of biuret solution for quantitatively measuring protein concentrations through spectrophotometry. It is established that biuret reagent can indicate the presence of proteins by a color change, with the intensity of this change being proportional to the protein amount. The optimal wavelength for measurement is 450 nm. While biuret is effective, it is noted that it may lack the sensitivity of the Bradford reagent for protein quantification.

PREREQUISITES
  • Understanding of protein chemistry and peptide linkages
  • Familiarity with spectrophotometry techniques
  • Knowledge of colorimetric assays for protein measurement
  • Experience with laboratory reagents, specifically biuret and Bradford reagents
NEXT STEPS
  • Research the principles of spectrophotometry and its application in protein quantification
  • Explore the differences in sensitivity between biuret and Bradford assays
  • Learn about the preparation and use of biuret reagent in laboratory settings
  • Investigate alternative methods for protein measurement, such as Lowry or BCA assays
USEFUL FOR

This discussion is beneficial for biochemists, laboratory technicians, and researchers involved in protein analysis and quantification methods.

jen333
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hi all!
I was just thinking about organic compound indicators, and biuret solution for proteins crossed my mind. I know that it can be used to qualitatively tell the presence of proteins (peptide linkages) with a colour change. But is there a way that biuret solution can be used to take quantitative measurements? ie) spectrophotometry, or is the reaction exothermic allowing a temperature measurement (not quite sure)
Hope to receive some thoughts! thx!
 
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Just did a quick search and while the papers I pulled up were somewhat old, mostly 70s/80s, Bieuret can be used with spectrophotometry. The intensity of the colour change is proportional to the amount of protein present.
 
In my old lab, we used biuret reagent to measure protein concentration of a solution rather than the bradfort reagent. You measure the change in colour using a spectrophotometere. I think the wavelength is 450 nm.

However, the biuret reagent may not be as sensitive as the bradfort, but I'm 100% sure.
 

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