Using Biuret Solution to Quantitatively Measure Proteins

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Biuret solution is primarily known for its qualitative ability to indicate the presence of proteins through a color change due to peptide linkages. It can also be utilized for quantitative measurements via spectrophotometry, where the intensity of the color change correlates with protein concentration. The typical measurement wavelength for this method is around 450 nm. While biuret reagent is effective for measuring protein concentration, it may not be as sensitive as the Bradford reagent, which is often preferred in laboratory settings.
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hi all!
I was just thinking about organic compound indicators, and biuret solution for proteins crossed my mind. I know that it can be used to qualitatively tell the presence of proteins (peptide linkages) with a colour change. But is there a way that biuret solution can be used to take quantitative measurements? ie) spectrophotometry, or is the reaction exothermic allowing a temperature measurement (not quite sure)
Hope to receive some thoughts! thx!
 
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Just did a quick search and while the papers I pulled up were somewhat old, mostly 70s/80s, Bieuret can be used with spectrophotometry. The intensity of the colour change is proportional to the amount of protein present.
 
In my old lab, we used biuret reagent to measure protein concentration of a solution rather than the bradfort reagent. You measure the change in colour using a spectrophotometere. I think the wavelength is 450 nm.

However, the biuret reagent may not be as sensitive as the bradfort, but I'm 100% sure.
 
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