Hi, I have a movie that contains a particle moving from one end of a channel to another end. The velocity of the particle is super-fast. I need to know how I can estimate the time and pictorially (like using a histogram--similar to plotting the heart pulses of a person) be able to tell when the particle is at each of the ends....I don't care too much about the intermediate path. I have started using the image processing toolbox on matlab...I'm thinking that my steps will be- 1. to view the movie on movie player (on the image processing toolbox) 2. split the video into individual frames. 3.attach a histogram to each frame, to tell (due to difference in light intensity in the area where the particle is located) in which part of the frame the particle is. 4. now I can tell the time difference between the two frames in which I can see the particle is at each end. So does anyone have a better idea on how I can achieve my goal? What functions will I have to use?