Practically, what the square root on the bottom does is two things:
1) It limits where the function can be defined. If x4>81, then the function is not defined. It also has the potential to make asymptotic behavior different... an example is something like
[tex]\frac{x-1}{ \sqrt{(x^4-1)(x-1)}}[/tex]
At first glance it appears to have an asymptote at x=1 and x=-1, but the x-1 at the top cancels with the x-1 in the denominator (you can see this by factoring the denominator) in the limit as x approaches 1, so no asymptotic behavior occurs.
What's required for a zero in the denominator to cause a vertical asymptote is for there to be no canceling zeroes, or not enough canceling zeroes at least, in the numerator. Having a square root can effect this as it effectively "halves" the power of the zero in the denominator.
You definitely have at least one more vertical asymptote. For what x is x4-81=0? You should be able to fully solve this (either by factoring or by noticing you can set x2=y and solving y2-81=0 first)