What is a duplicate sample when preforming a photometric protien assay

[vande060]

what is a"duplicate sample" when preforming a photometric protien assay

Homework Statement

Q: How do you prepare duplicate samples? Why is it important to run samples in duplicate of triplicate?


what is a"duplicate sample" when preforming a photometric protien assay?

Homework Equations

The Attempt at a Solution

When we did the BCA analysis of bovine serum albumin, or professor asked us this question. We went ahead and did serial dilutions for the same but we only did one set of them. Is a duplicate just the same set of dilutions done twice? So you are more or less double checking to make sure the dilutions were right and all had the same absorbances?

 

[Nino MMP]

A duplicate sample is when the same sample is tested multiple times in a photometric protein assay. This is done to ensure accuracy of results and to minimize potential errors. Duplicate samples should have similar results, if they differ significantly then it may be an indication of an error in the experiment. It is important to run samples in duplicate or triplicate to verify the accuracy of the results and reduce the chances of errors.