what is a"duplicate sample" when preforming a photometric protien assay 1. The problem statement, all variables and given/known data Q: How do you prepare duplicate samples? Why is it important to run samples in duplicate of triplicate? what is a"duplicate sample" when preforming a photometric protien assay? 2. Relevant equations 3. The attempt at a solution When we did the BCA analysis of bovine serum albumin, or professor asked us this question. We went ahead and did serial dilutions for the same but we only did one set of them. Is a duplicate just the same set of dilutions done twice? So you are more or less double checking to make sure the dilutions were right and all had the same absorbances?