What Is Polyclonal Antiserum? Answers Here

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Polyclonal antiserum consists of a mixture of antibodies produced by different B cell lineages in response to an antigen. These antibodies recognize multiple epitopes on the antigen, providing a broader immune response compared to monoclonal antibodies, which target a single epitope. The process of obtaining polyclonal antiserum involves immunizing an animal with the antigen, followed by isolating the blood to extract serum containing the antibodies. The serum can be further purified using techniques like protein A or protein G to enhance its specificity and effectiveness. This makes polyclonal antiserum a valuable tool in various applications, despite being less specific than monoclonal antibodies.
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What is polyclonal antiserum? I have checked the net, but could not find any good explanation on it.

Thanks.
 
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You should be looking for polyclonal antibodies: they recognize multiple epitopes, making them more robust to small changes in the antigen. Monoclonal antibodies react only to one epitope, making them highly specific, but they are more expensive and take longer to develop.
 
So polyclonal antiserum is the same as polyclonal antibodies? :rolleyes:
 
You immunize an animal with an antigen, after which you isolate blood and get serum from that. That is your antiserum that will contain antibodies to the antigen. You can purify the serum with protein A, or protein G, depending on the chemistry.
 
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