What Is the Difference Between Non-Reducing & Reducing Buffers?

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The discussion focuses on the differences between non-reducing and reducing buffers in biochemical contexts. Reducing buffers contain agents like DTT that facilitate the reduction of disulfide bonds in proteins, which is crucial in maintaining protein structure in a reducing environment, such as the cytoplasm of a cell. In contrast, non-reducing buffers do not contain these agents, allowing disulfide bonds to remain intact. The importance of redox reactions, particularly in cellular environments, is emphasized, highlighting how reducing conditions affect protein folding and stability.
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what is the difference between a non-reducing and a reducing buffer? :rolleyes:

and what does it mean with non-reducing and reducing?

thanks a bunch!
 
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Are you familiar with redox reactions? Reduction and Oxidation. The cytoplasm of a cell is a reducing environment: an effect is that disulfide bonds within proteins get reduced and are thus hard to form.

DTT is a reducing agent and often added to reducing buffers.
 
thanks Monique! it seems like you know a lot of things :smile: are you a PhD?
 

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