What kind of errors would be produced by excess taq in PCR?

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Excess Taq polymerase in PCR can lead to several issues, primarily due to the enzyme's storage in glycerol, which can inhibit the reaction. Adding too much Taq, such as 6.75µL to a 50µL cDNA mix, may result in decreased efficiency and specificity of amplification. The recommended practice is to limit enzyme addition to no more than 10% of the total reaction volume to avoid these negative effects. Using only a portion of the mixture for reactions does not mitigate the potential problems caused by the initial excess of Taq polymerase.
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What kind of errors would be produced by "excess" taq in PCR?

Not sure if this belong in the Homework section. Put it here since it's not homework.

What kind of errors will I get by adding 6.75µL taq pol to +50µL of cDNA? The master mix will be added to the cDNA resulting in an excess of taq pol. Only 2 and 4µL of the taq+cDNA mixture will be used for each reaction not the entire +50µL.
 
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There is not information here to answer your question. As a rule you never add more than 10% of enzyme to your reaction, this is because your enzyme is stored in glycerol and that this is inhibitory to the reaction.
 

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