as vague as the question was, i think i may understand. After knowing electron flow of current and then going to school for automotive technology, i got totally lost when working with multi-meters and ohms law just because of the fact that us AUTOMOTIVE guys OBSERVE the CONVENTIONAL flow of current.
If you want to make sense of current flow/voltage using a multi meter, what took me a week to understand was the fact that when you place a lead on the on a part of the electrical system to check for voltage with the other lead on the negative side of the battery ( in most cases. your observing one direction of flow. as soon as you put that lead on the positive terminal as opposed to the negative, your voltage reading will change. Therefore observing a different side of the circuit. it all depends on your frame of reference. when you touch meter leads backwards, you get the same reading always, but with a (-) sign.
If you were to hook a car battery up backwards, at least nowadays, that could be potentially lethal to your cars power control module, alternator and so on/so forth. this is strictly because of the fact that these ELECTRONIC things have diodes and transisters that are current flow direction-specific. (allows flow one way, blocks flow another way.) The reason you can hook a flashlight up backwards (incandescent light), is because there are no electronics involved.
This should explain why the direction of flow matters and where it is significant, even the electrical engineers in the auto industry all know to observe this conventional flow and design electronics with respect to the power and ground sides of the electrical system.
In a nutshell, this is the significance of flow direction.