Calculate % Excitation of Sample X w/ Laser Pulsed Source

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To calculate the energy required to excite 5% of sample X with a pulsed laser at 532 nm, one must first understand the relationship between the laser's power, repetition rate, and energy. The energy can be calculated using the formula E = power/rep.rate, which helps determine the number of photons using E = Nhc/lambda. The absorption coefficient of the sample, given as 9.895e-18 cm² molecule⁻¹, is crucial for understanding how much energy is absorbed. Additionally, fluence can be calculated by measuring the laser spot size when focused on the sample. Understanding these calculations will aid in determining the appropriate energy or power needed for excitation.
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Hi Everyone,

I am new to this forums. I am not sure where to post but general discussion is a good place. I am trying to figure out a problem:

I have a sample X in gas phase. Say it has an absorption coefficient of 9.895e-18 cm2 molecule-1 @ 532nm. I have a laser pulsed source (say 50 Hz) giving me 532nm light. How does one calculate what energy one needs to excite 5% or any percentage of the sample? The measured power of the pulsed laser is related to the energy by:

E=power/rep.rate

and one can use this to calculate the number of photons N,by

E=Nhc/lambda where lambda is the wavelength (in the example above it is 532nm).

we can also calculate the fluence by measuring the spot size as we focus the laser beam into the sample. I can't figure out how people choose the energy/power from absorption cross section calculations? I am a novice, so need a little help please. Thank you.

sciphys1
 
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