CHEF Gel Troubleshooting: Help With Plugs & Ladder

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The discussion centers on issues encountered while running a CHEF gel, specifically the failure of DNA plugs to migrate out of the wells. The user reports seeing a thick band in the well during ethidium bromide staining and mentions the absence of a ladder. Responses emphasize the importance of checking the power supply, suggesting the use of a voltmeter to ensure proper function. If the power supply is pulsed, an oscilloscope may be necessary for troubleshooting. The conversation highlights the need to rule out power supply issues as a primary step in resolving the migration problem.
MatthewHaas
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Has anyone ever ran a CHEF gel before? I am having trouble getting my plugs to leave the wells. Ethidium bromide staining shows a thick band in the well, and I am supposed to have a ladder. I have some images I can post, but I figured it makes more sense to first see if anyone out there has any expertise in the area.

Thanks!
 
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Did anything get out of the well? I assume that you have already ruled out power supply issues, yes?
 
I am sorry for the late reply. No, nothing got out of the well. We have not rulerd out power supply issues.
 
Power supply is the first thing you check. Use a voltmeter unless the power supply applies a pulsed potential. If it is pulsed, you need an oscilliscope.
 

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