Designing Primers: Finding Software to Test Primers

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The discussion centers around the search for software to test DNA primers after the user switched workplaces and lost access to the Amplify program, which visualizes primer efficiency through virtual PCR products. The user expresses a preference for Amplify over other options, specifically mentioning Primer3 from MIT, which they do not favor. They successfully locate Amplify online but note their lack of a Mac to use it. Additionally, they mention DNA Stryder as a good software for sequence manipulations, although it is only available for Mac users. The user highlights cellbiol.com as a valuable resource for downloading software, including freeware for PCs.
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Now I am looking for some software to test primers with. I always used to eye-ball a sequence, and based on that pick some good looking sequences, put it into the Mac program: Amplify.

This program then visualizes how specific and efficient the priming is on the target sequence by virtual pcr products.

BUT changed workplace, no Amplify.. I am trying to find it on the net though.

Does anyone now other programs which can handle the task? PC or Mac operatable?

I know of primer3 from MIT, but I don't like it..
 
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I have found the Amplify program!

http://www.cellbiol.com/soft.htm

too bad that I don't have a Mac :S

The DNA stryder is really good software too, for sequence manipulations, BUT only for Macs..
 
Tadaaaa!

Freeware for PCs
http://www.cellbiol.com/modules.php?op=modload&name=Downloads&file=index&req=viewsdownload&sid=5


Pretty cool site, cellbiol.com
 
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