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Biology questions

  1. Jul 2, 2009 #1
    1. The problem statement, all variables and given/known data

    Which of the following methods is suitable to sterilize a liquid medium,containing blood serum?
    (1) pasteurization (2) Autoclaving at 121degrees celsius for 10 minutes (3) Boiling at 100 degree celsius for 10 minutes (4) Filtration using a sterile membrane filter (5) Freezing at -20 degrees celsius
    Ans given is (2)
    2. Relevant equations
    n/a



    3. The attempt at a solution
    I don't understand why it can't be (4).Why can't we use a sterile filter to sterilize blood serum,afterall sterilization is to remove or kill any microorganisms present in a medium,right?
    My other question is,isn't blood serum a thermolabile medium so how can we sterilize it by heating it,without denaturing any of the plasma proteins present?
     
  2. jcsd
  3. Jul 3, 2009 #2
    What is in blood serum? What is it used for? Are you sure you want to filter everything away?

    As for denaturation, yes some of that will happen, but I think you can be sure the temperature and time for autoclaving is carefully chosen and optimized for its purposes.
     
  4. Jul 4, 2009 #3
    I didn't think of it that way.
    Thanks.now I understand,why we can't use a filter.
     
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