The discussion centers on RNA splicing software, specifically tools used to predict RNA splicing sites. A notable recommendation is GeneSplicer, available through the Institute for Genomic Research (TIGR). Participants express a desire for software that identifies splicing sites without separating introns and exons, as there is a belief that splicing occurs within introns. It is noted that while knowing intron and exon locations can help deduce splice sites, existing software may not be entirely accurate, particularly for initial predictions. The conversation suggests that if alternative splicing is suspected, conducting molecular biology experiments may be necessary for validation.