Depth of Field (DOF) for a lens is defined as the maximum allowable defocus for a clear Fraunhofer Pattern, but it is not a fixed property since perfect focus occurs at a single plane. The DOF is influenced by the lens's f-number or numerical aperture (NA), with a general formula of DOF = 2 * wavelength * n / NA^2 in the paraxial limit. For lenses with larger numerical apertures, such as those used in microscopy, this formula is adjusted. Additionally, achieving the Rayleigh criterion requires aberrations to be less than one-quarter wavelength. Understanding these principles is crucial for applications requiring precise optical performance.