- #1
melodyh
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if sample we have is .49mg, how much 100ul/ml solution can one make?
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How to Create a 100ul/ml Solution Using a Sample of .49mg?
- Thread starter melodyh
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In summary, a dilution is a process of reducing the concentration of a solution by adding more solvent. This is done by dividing the volume of the final solution by the volume of the initial solution to calculate the dilution factor. Concentration is the measure of the amount of solute present in a given volume of solution, and to prepare a solution with a specific concentration, you will need to use the formula C1V1 = C2V2. Some common dilution errors to avoid include using the wrong measurements, not properly mixing the solution, and not accounting for changes in volume due to temperature. It is important to carefully follow the dilution protocol and double check all measurements to avoid errors.
- #2
Borek
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No idea what you are asking about 
Please elaborate.
Please elaborate.1. What is a dilution?
A dilution is a process of reducing the concentration of a solution by adding more solvent. This results in a decrease in the number of solute particles per unit volume, thus lowering the concentration of the solution.
2. How do I calculate a dilution factor?
A dilution factor is calculated by dividing the volume of the final solution by the volume of the initial solution. For example, if you add 5 mL of a solution to 15 mL of water, the dilution factor would be 15/5 = 3.
3. What is a concentration?
Concentration is the measure of the amount of solute present in a given volume of solution. It is usually expressed in units of moles per liter (molarity) or grams per liter (mass concentration).
4. How do I prepare a solution with a specific concentration?
To prepare a solution with a specific concentration, you will need to know the molarity or mass concentration of the solute and the desired volume of the solution. You can then use the formula C1V1 = C2V2, where C1 is the initial concentration, V1 is the initial volume, C2 is the desired concentration, and V2 is the desired volume.
5. What are some common dilution errors to avoid?
Some common dilution errors to avoid include using the wrong volume or concentration measurements, not properly mixing the solution, and not accounting for any changes in volume due to temperature. It is important to carefully follow the dilution protocol and double check all measurements to avoid errors.
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