Ethanol production from sugar using yeast is limited by the alcohol tolerance of the yeast strain, which typically dies around 13% alcohol concentration. While some more resilient bacteria may survive, once the yeast is dead, ethanol production ceases. To potentially increase yield, distilling ethanol during fermentation can prevent high alcohol buildup. The specific yeast strain used can also influence the maximum alcohol concentration tolerated, with some strains capable of surviving higher levels. A comparison chart of yeast strains can provide additional insights into their alcohol tolerance.