Hey guys, gals (Monique ) Quick question. I recently completed a labortatory session in my Genetics class regarding the isolation and analysis of plasmid DNA, and a section of the report has me stumped. Before the plasmid DNA was put under the spectrophotometer, it was diluted in 285µl of distilled water (15µl of DNA, all up 300µl). Absorbance was measured, and found to be around 0.2 (Don't have the exact figures on me). Now assuming A=1 is the equivelant of 50µg/ml, we need to calculate the concentration of the plasmid DNA sample, taking into consideration the dilution. I realise this is simple maths, but for some reason when it comes to facotring dilutions into my calculations, my brain laughs at me and tells me where to go. So I need your help guys :) Thanks in advance.