How can I accurately measure the density and volume of microdroplets in oil?

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SUMMARY

The discussion focuses on measuring the density and volume of aqueous microdroplets in oil. The initial method proposed involves weighing a known volume of the droplet-in-oil mixture and comparing it to the weight of the oil alone to estimate droplet density. Challenges arise in accurately estimating droplet volume, prompting suggestions such as using transparent plates for droplet counting under a microscope or centrifuging the oil to separate droplets. These methods depend significantly on the size of the droplets.

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I need to measure the density of aqueous microdroplets in oil. My first idea was to obtain a set volume of the droplet-in-oil mixture and weigh it to obtain the density, weigh the same volume of oil without droplets, then estimate the volume of the droplets so I can work out their density.

Estimating the volume of the droplets is turning out to be trickier than expected.

Does anyone have any ideas as to how I can measure the density and / or volume of microdroplets in oil?

Thank you
 
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How large are the droplets (rough estimate)?

Can you take a small (known) volume and squeeze it between two transparent plates to make the droplets countable with a microscope?
 
Or maybe you could centrifuge the oil to make the droplets go on the surface. But it all depends on the size. mfb already asked.
 

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