How to Calculate Hemolysis Percentage from Absorption at a Given Wavelength?

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Calculating hemolysis percentage from absorption at a specific wavelength involves understanding the relationship between light absorption and NaCl concentration. The formula stating that the absorbance of the most hypotonic solution equals 100% hemolysis raises confusion, as it suggests that hemolysis is directly linked to NaCl concentration. The discussion highlights that hemolysis may depend on various factors, including the type of cells and their absorption spectrum, particularly the hemoglobins involved. Overall, clarity on how NaCl concentration influences light absorption and hemolysis is essential for accurate calculations.
ChrisVer
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How can someone calculate the hemolysis (%) from the absorption at λ wavelength given some concentration in NaCl?

The formula I saw was really weird:
Absorb(most hypotonic solution) = 100 % hemolysis
I canlt understand that formula though... thanks
 
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Whoa. This seems a bit lacking in basic information. First off, hemolysis may be a function solely of NaCl concentration, as well, for some types of cells. What you indicate seems to say that the effect of wavelength of light is changed by NaCl concentration. Uhh, where did you get this? As is, I cannot help you. If you can't tell, I'm totally confused.

I would assume that light induced hemolysis is at least in part dictated by the absorption spectrum of the hemoglobins involved, for example.
 

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