How to prepare 50mM Na2HPO4/H3PO4 buffer, pH 4.5

AI Thread Summary
To prepare a 50mM Na2HPO4/H3PO4 buffer at pH 4.5, it's important to understand that the "/" indicates a mixture of both compounds. The discussion highlights that this buffer choice may not be ideal for pH 4.5. A suggested method involves preparing H3PO4 and then neutralizing it with NaOH to form Na2HPO4. Alternatively, mixing the acid and salt solutions can also achieve the desired buffer. Understanding buffer solutions is crucial for proper preparation.
Natsu1993
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The have to prepare the buffer but I really don't know how. Is the any formula for the buffer preparation?

Ps. Sorry but one more thing, what does the "/" between Na2HPO4 and H3PO4 mean?
 
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/ means buffer containing both Na2HPO4 and H3PO4.

I strongly suggest you start by reading about buffer solutions, for example here: https://en.wikipedia.org/wiki/Buffer_solution (but there are plenty of other resources on the web).

For pH 4.5 this is a very bad buffer choice.

Sorry to say that, but from the way you asked the question it is clear you are not qualified for the task.
 
So, I have to prepare H3PO4 and then add NaOH?

H3PO4 + 2NaOH ---> Na2HPO4 + 2H2O

Am I correct?
 
This is one of ways of doing it. You can as well mix the acid and the salt solution.
 
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