HPLC regarding the pore size vs the molecule size

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In HPLC (High-Performance Liquid Chromatography), the relationship between pore size and analyte molecule size is crucial for effective separation. Generally, having a pore size smaller than the analyte molecule size can lead to faster elution of the analyte, as it may not penetrate the stationary phase effectively. This can result in the analyte being eluted before the solvent. However, this setup is not typical, as it can hinder the separation process. The discussion highlights a need for clarity on HPLC mechanisms and the implications of pore size on analyte behavior. Further insights or recent findings on this topic are sought to enhance understanding.
kl055
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Hi

I have a simple question about HPLC regarding the pore size vs the molecule size of the analyte.

Are there cases when it is desirable to have a pore size smaller than the analyte molecule size?

In this case, the analyte will be eluted first, then the solvent will be eluted later?

Do I misunderstand how HPLC works?
 
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