Isothermal Settling (aka sedimentation equilibrium)

In summary, the conversation discusses the terms "isothermal" and "settling" and their significance in experiments. The definition of isothermal is explained as a consistent temperature throughout, while settling refers to the movement of heavy particles towards the bottom. The group debates whether a cell can be considered isothermal and discusses the possibility of isothermal settling occurring within cells. They also mention the potential impact of endothermic/exothermic reactions on local temperature variations within the cell.
  • #1
twall11347243
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1
Is anyone familiar with these terms or the experiments involved? I have a lot of questions regarding them. Thank you guys.
 

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  • #2
"Isothermal" simply means that its one temperature from top to bottom.
Settling is what happens to heavy particles in the liquid. Not surprisingly, big heavy particles drop like a rock and end up at the bottom (H=0). Others take more time and fall onto the accumulation of bigger particles.
 
  • #3
Okay, so would you consider the cell to be 'isothermal'? I don't, but apparently it is according to this Paul Todd paper (See attached).
Overall the cell may be 'isothermal' to maintain a constant 37 oC... but within the molecular environment there endothermic/exothermic reactions where heat is being transferred and local temperature variations occur due to increases in kinetic energy.

He mentions this isothermal settling is a process to be considered inside cells. What do you think about that? Isothermal settling is a static state of equilibrium of no net mass movement, with more particles concentrating towards the bottom. I wouldn't think cells would be able to survive under these conditions.
 

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What is isothermal settling?

Isothermal settling, also known as sedimentation equilibrium, is a process in which particles suspended in a liquid settle down and reach a state of equilibrium under constant temperature.

What factors affect isothermal settling?

The rate of isothermal settling can be affected by factors such as particle size, density, and shape, as well as the viscosity and density of the liquid medium.

How is isothermal settling measured?

The rate of isothermal settling can be measured using techniques such as centrifugation or sedimentation velocity analysis, where the particles are separated based on their sedimentation rate.

What is the importance of isothermal settling in scientific research?

Isothermal settling is an important process in various fields of science, including biology, chemistry, and environmental science. It is used to study the properties of particles and their interactions in different systems.

What are the applications of isothermal settling?

Isothermal settling has various applications, such as in the purification of proteins, separation of different types of particles, and determination of particle size and shape. It is also used in industries such as pharmaceuticals, food and beverage, and wastewater treatment.

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