Weight to pmol/l for nucleotides

[sotellme]

How can i transform weight into pmol/l when i have to prepare the primers?


Which formula should i use to know this transformation?

The protocol; Dissolve dried primer stocks at 100 pmol/l in RNAse-free water and store at -20°C.



Thanks.

 

[Moonbear]

You need to know the sequence of your primers, then it's just basic chemistry where you calculate your molecular weight to determine molarity.

 

[sotellme]

What if my primer has 6 nucleotides do you know how i can calculate into 100 pmol/l?

Thanks.