What are the limitations of RP-HPLC in chemical analysis?

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HPLC, while widely used in chemical analysis, has notable limitations. It can only analyze compounds that are soluble in the mobile phase, which restricts its applicability to certain substances. Additionally, HPLC is unsuitable for compounds that react with either the mobile phase or the stationary phase, further narrowing the range of analyzable mixtures. These constraints highlight the need for alternative methods when dealing with insoluble or reactive compounds.
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As HPLC is such a common method in chemical analysis, I was just wondering what are the limitations to such a technique? What compounds/mixtures is it limited to analyzing?
 
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Well it certainly is limited to the analysis of compounds which are soluble in the mobile phase. It is limited to the analysis of compounds which are not reactive with the mobile phase or stationary phase as well.
 

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