What Determines the Resolution of Different Microscopy Techniques?

[Gogsey]

I have a question about different microscopy techniques. What is major factor in determining a microscope's lateral and axial resolution for these three types:

Confocal
Multiphoton
OCT

The questions says to choose between wavelength, beam diameter, spot size, focal length, and depth of focus.

I so far I say the following for each type:

Confocal: Spot Size
Multiphoton: Depth of focus or focal length, not to sure about this one
OCT: Wavelength

Can anyone help me out or confirm these are correct.

Thanks

 

[NateD]

!Your answers are mostly correct. However, for multiphoton microscopy, the resolution is determined by a combination of wavelength, depth of focus, and beam diameter. In OCT, the resolution is determined by wavelength and spot size.