The discussion centers on the concept of atomic mass units (amu) and their relation to the mass of protons, neutrons, and electrons. One amu is defined as one-twelfth the mass of a carbon-12 isotope, approximately equal to 1.66 x 10^-24 grams. The mass of an oxygen atom, which is 16 amu, does not directly correspond to 16 protons, neutrons, or electrons due to the binding energy in the nucleus affecting the total mass. Participants emphasize the importance of researching basic scientific concepts, suggesting that simple questions could be resolved through online resources or textbooks. The conversation also critiques the frequency of basic questions posed by one user, questioning their understanding given their academic background.