Leaving Italian bread out on the counter for a few hours leads to hardening primarily due to evaporation. While there is a potential for microbial growth on sugar sources at room temperature, significant spoilage typically requires 24 to 48 hours for bacteria and 3 to 4 days for fungi to develop. In the short span of three hours, the bread hardens mainly from moisture loss rather than microbial activity. To maintain softness, it's recommended to store bread in a bag. If the bread does harden, reheating it in the oven or toasting can improve its taste and texture.