Can Software Predict RNA Splicing Sites?

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The discussion centers on RNA splicing software, specifically tools used to predict RNA splicing sites. A notable recommendation is GeneSplicer, available through the Institute for Genomic Research (TIGR). Participants express a desire for software that identifies splicing sites without separating introns and exons, as there is a belief that splicing occurs within introns. It is noted that while knowing intron and exon locations can help deduce splice sites, existing software may not be entirely accurate, particularly for initial predictions. The conversation suggests that if alternative splicing is suspected, conducting molecular biology experiments may be necessary for validation.
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RNA Splicing Software ?

What is the Softwares used to predict RNA splicing sites ?
 
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I don't know which ones are regarded the best, but http://www.tigr.org/tdb/GeneSplicer/gene_spl.html" is a software program freely available through the Institute for Genomic Research (TIGR).
 
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Hey thanks :)

But, i don't want anything which sequesters introns and exons.

Coz i suspect that splicing takes place inside INTRON.
So software has 2 identify only the site of action and not separating introns and exons !
 
? but if you know where the introns and exons are, you can automatically deduce the splice site.. and these software programs are not perfect in predicting splicing, especially the first one is difficult to get correct I believe.

If you suspect alternative splicing is taking place, I'd do some molecular biology experiments..
 
https://www.discovermagazine.com/the-deadliest-spider-in-the-world-ends-lives-in-hours-but-its-venom-may-inspire-medical-miracles-48107 https://en.wikipedia.org/wiki/Versutoxin#Mechanism_behind_Neurotoxic_Properties https://www.sciencedirect.com/science/article/abs/pii/S0028390817301557 (subscription or purchase requred) The structure of versutoxin (δ-atracotoxin-Hv1) provides insights into the binding of site 3 neurotoxins to the voltage-gated sodium channel...
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