Raman Microscopy: Best Way to Hold Micron-sized Polystyrene Beads

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The discussion focuses on the optimal methods for holding micron-sized polystyrene beads during Raman microscopy. It highlights the use of laser tweezers for optical trapping, which can automatically hold particles in place while still requiring microscope slides for support. References to relevant literature, such as D.V. Petrov's work on Raman spectroscopy and optical traps, emphasize the established techniques in the field. The conversation indicates a need for practical solutions in experimental setups involving these particles. Overall, effective sample handling is crucial for successful Raman microscopy of micron-sized beads.
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What's the best way to hold a sample of micron-sized polystyrene beads for raman microscopy?
 
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With a laser tweezer:

Raman spectroscopy of optically trapped particles
D V Petrov 2007 J. Opt. A: Pure Appl. Opt. 9 S139-S156 doi: 10.1088/1464-4258/9/8/S06
 
Already know about that...seems I'm going to have to use microscope slides...
 
After reading that article and other articles regarding optical traps and microscopy, it seems that the optical trapping is an automatic process? Ie. you still need the cover slides but the particles are automatically trapped by the beam.

"Optical Trapping and Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy of Submicron-Size Particles"
IEEE JoQE Vol.11 No. 4 Jul/Aug 2005
 

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